Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Doublecortin-like Kinase 1 (also known as DCLK1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse DCLK1 antibody. DCLK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse DCLK1 Antibody is added and binds to DCLK1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DCLK1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse DCLK1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm